Glossary of Terms
Anemia: A medical condition caused by low concentrations of red blood cells and hemoglobin resulting in a reduced oxygen carrying capacity of the blood.
Bloodless surgery: Any surgery performed with out the use of transfused blood.
Dialysis: A blood cleaning/filtering process performed on individuals with failing kidneys.
DNA ligase: An enzyme capable of repairing breaks in the DNA backbone by forming the phosphodiesterbond that is placed between the 5' phosphate and the 3'OH group of ribose.
Expression vector: A cloning vector that contains regulatory sequences that promote transcription and translation of a cloned gene.
Glycoprotein: A category of macromolecules in which a single polysaccharide or group of polysaccharides are combined with a protein.
Hematocrit: The portion of blood volume consisting only of red blood cells.
Hemoglobin: A protein complex found in red blood cells, composed of two proteins (alpha and beta) that bind molecular oxygen for transport through out the body.
Hypoxia: A physiological condition where the body's requirements for oxygen are not satisfied.
Plasmid: Extrachromosomal genetic material that is not essential for growth and has no extracellular form.
Recombinant DNA: A molecule of DNA consisting of DNA originating from two or more sources.
Restriction
enzyme: Enzymes that are capable of recognizing and cleaving
specific DNA sequences (usually 4, 6 or 8 bases in length) that result
in a single-stranded end. Over 2000 restriction enzymes, with over
200 restriction sites, are known.
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